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AmScope IN300TC-FL-MF Digital Long Working Distance Inverted Fluorescence Trinocular Microscope, 40x-1000x, WH10x and WH25x Eyepieces, Phase-Contrast Objectives, 30W Halogen Illumination, 0.3 NA Abbe Condenser, Mechanical Stage, 110V, Includes 1.3MP Fluorescence Video Camera and Software

Inverted epifluorescence microscope with mercury lamp light source for performing sample analysis of biological specimens, sedimentary rock, and semiconductor impurities, and 1.3MP fluorescence video camera with USB 2.0 output for capturing and displaying still and live video images on a monitor or TV
Trinocular head with Siedentopf binocular mount, interchangeable pairs of 10x and 25x high-eyepoint super-widefield eyepieces, 50 to 75mm interpupillary adjustment, and fixed 45-degree vertical inclination; vertical port accepts C-Mount or 23mm camera
Under-mounted quadruple nosepiece with 4x, 10x (phase-contrast), 20x (phase-contrast), and 40x DIN long working-distance plan objectives that provide a longer working distance and improved focus over the entire viewing field

The AmScope IN300TC-FL-MF digital inverted epifluorescence trinocular microscope has interchangeable pairs of 10x22mm and 25x10mm high-eyepoint super-widefield eyepieces, an under-mounted quadruple nosepiece with four DIN long working-distance plan objectives, fluorescence 100W HBO mercury lamp and Brightfield 30W halogen illumination, a 0.3 NA Abbe condenser with iris diaphragm, and a double-layer mechanical stage with a stage stop to give protection to slides and objectives from damage. The 1.3MP fluorescence camera has a CMOS color sensor, image capture and editing software, and USB 2.0 output to capture or display still or video images on a pc or projector. The anti-mold trinocular head has a Siedentopf binocular mount with 50 to 75mm interpupillary adjustment and a fixed 45-degree vertical inclination to reduce eye and neck strain. A Siedentopf binocular head enables the viewer to change the interpupillary distance without changing the tube length, getting rid of the wish to refocus the image. Anti-mold coatings give protection to the microscope in high-humidity environments. Interchangeable pairs of WH10x22mm and WH25x10mm high-eyepoint super-widefield eyepieces provide improved focal point over all the field of view (FOV). High-eyepoint eyepieces ease viewing for viewers who wear glasses. Dioptric adjustment on both eyepieces incorporates individual eye-strength differences. The vertical trinocular port accepts a 23mm or C-Mount camera. The under-mounted nosepiece has 4x, 10xPH (phase-contrast), 20xPH (phase-contrast), and 40x (spring) DIN long working-distance plan objectives that combine with the eyepieces to offer color correction of magnified images. Long working-distance plan objectives provide improved focal point over all the range of the viewing field and a longer working distance that may be required when viewing specimens in dishes and large containers. Phase-contrast provides high contrast and visibility without the usage of stains, allowing specimens to be observed of their natural state without being killed or fixed. A digital inverted microscope is utilized in research, medical, and industrial laboratories for remark of biological specimens within vials or dishes that require more vertical working space, and where image capture, detailed records, or documentation is required.

The included AmScope MT1400-CCD 1.3MP fluorescence camera has a 1/3″ Sony color CMOS sensor for displaying still microscopy images and streaming live videos to a pc monitor or digital TV. The camera is suitable for low-light applications, including fluorescence and darkfield microscopy. The camera can also be mounted in any standard C-Mount. The sensor has an interline scan mode, an RGB Bayer pattern filter, and a 280mV G-sensitivity. The camera includes image capture and editing software that provides still image and live video capture and editing capability, including measurement functions. User-defined parameters include brightness, gain, automatic or manual exposure selection, RGB, white balance, and light angle. Exposure duration has a range of 0.1 to 60 minutes. The software supports JPG, TIFF, GIF, PSD, WMF, and BMP file formats and is compatible with Windows XP, Vista, 7, and 8. The camera supports Twain, DirectShow, Amcap, and Minisee file formats. The camera has a USB 2.0 data port (cable included). The camera includes an aluminum case to give protection to the camera all the way through storage or transport.

Brightfield, phase-contrast, and fluorescence illumination is mounted above the stage and has transmitted 30W halogen illumination. Brightfield (BF) illumination allows the specimen to soak up light, resulting in a dark image on a light background. The phase-contrast element aligns the light source with the phase-contrast objectives to optimize high contrast and visibility without the usage of stains, allowing specimens to be observed of their natural state without being killed or fixed. Halogen illumination provides bright light in a concentrated path, and a rheostat controls the amount of light emanating from the lamp. Frequently utilized in biological and metallurgical research, fluorescence microscopy uses a xenon or mercury lamp to create ultraviolet light and excitation filters to filter unwanted wavelengths, allowing users to see details undetectable to other light sources. The movable 0.3 NA Abbe condenser can also be adjusted to regulate the distance of the light from the stage, and the iris diaphragm has an opening that focuses and controls the amount of light illuminating the specimen from fully open to fully closed. The double-layer mechanical stage with 1mm stage divisions and 0.1mm vernier resolution locks the slide into place and provides precise slide manipulation along the X- and Y-axes to allow coordinates to be recorded, enabling the viewer to go back to a specific location at the slide. The oversize stage is 9.53 x 6.77 inches (242 x 172mm) (W x D, where W is width, the horizontal distance from left to right; D is depth, the horizontal distance from front to back). Nested coaxial coarse and fine focal point has tension-adjustable coarse focusing and an adjustable lock ring stopper that limits stage range to give protection to slides and objectives from damage. All-metal mechanical parts, solid-metal frame construction, and a stain-resistant enamel finish provide durability.

Microscope Specifications
Head Siedentopf trinocular
Magnification range 40x-1000x
Trinocular port 23mm or C-Mount
Eyepieces (30mm) WH10x20mm and WH25x10mm high-eyepoint super-widefield plan
Objectives (20mm) 4x, 10xPH, 20xPH, and 40x DIN long working-distance plan objectives
Stage Double-layer mechanical
Condenser Movable 0.3 NA Abbe with iris diaphragm
Light source Halogen with rheostat, 12V/30W
Illumination type Brightfield, phase-contrast, fluorescence
Power 110V, UL-listed

Camera Specifications
Resolution 1.3 MP
Image type Fluorescence
Display type PC monitor; Digital TV
Camera sensor 1/3″ Sony color Super CMOS, model IMX035
Exposure duration 0.1 to 60 minutes
Frame rate N/A
Mounting size C-Mount
Computer connection USB 2.0
File formats BMP, TIFF, JPG, PICT, PTL
Hardware compatibility PC
Software compatibility Windows 2000/XP/Vista/7
Power source USB 2.0

Microscopes are instruments used to toughen the resolution of an object or image. Types include compound, stereo, or digital. Compound microscopes use a compound optical system with an objective lens and an eyepiece. Stereo microscopes show object depth in a three-dimensional image. Digital microscopes are used to display an image on a monitor, reasonably than having a look through a lens. Microscopes may have monocular (one), binocular (two), or trinocular (three) eyepieces, with varying magnification abilities. Magnification ability refers back to the size of an image. Resolution, sometimes called resolvant power, refers back to the clarity of the image. The interaction between field of view (FOV), numerical aperture (NA), and working distance (WD) determines resolution. Microscopes can regulate magnification through a fixed focal point, or through a range of adjustments. They are able to also utilize LED, fluorescent, and mirror light sources to assist regulate viewing capabilities. Microscopes are widely utilized in education, lab research, biology, metallurgy, engineering, chemistry, manufacturing, and within the medical, forensic science, and veterinary industries.

United Scope manufactures microscopy equipment and accessories under the brand name AmScope. The company, founded in 1996, is headquartered in Irvine, CA.

What’s within the Box?

  • AmScope IN300TC-FL-MF microscope
  • WH10x20mm plan eyepieces, 30mm, one pair
  • WH25Sx10mm plan eyepieces, 30mm, one pair
  • 4x DIN long working-distance plan objective, 20mm
  • 10x DIN long working-distance phase-contrast plan objective, 20mm
  • 20x DIN long working-distance phase-contrast plan objective, 20mm
  • 40xS DIN long working-distance plan objective, 20mm
  • MT1400-CCD 1.3MP CMOS camera
  • (3) Color filters; yellow, blue, and green
  • Reflected illuminations system with top light housing and electrical cord
  • Episcopic light box and tube
  • Fluorescence Illumination power box
  • Mercury bulb, 100W
  • Halogen bulb, 30W
  • Protecting filter
  • USB cable
  • Software CD
  • Power cord
  • Dust cover
  • Instructions

Inverted epifluorescence microscope with mercury lamp light source for performing sample analysis of biological specimens, sedimentary rock, and semiconductor impurities, and 1.3MP fluorescence video camera with USB 2.0 output for capturing and displaying still and live video images on a monitor or TV
Trinocular head with Siedentopf binocular mount, interchangeable pairs of 10x and 25x high-eyepoint super-widefield eyepieces, 50 to 75mm interpupillary adjustment, and fixed 45-degree vertical inclination; vertical port accepts C-Mount or 23mm camera
Under-mounted quadruple nosepiece with 4x, 10x (phase-contrast), 20x (phase-contrast), and 40x DIN long working-distance plan objectives that provide a longer working distance and improved focal point over all the viewing field
Under-mounted 100W HBO ultra-high voltage spherical mercury lamp and upper-mounted rheostat-controlled 30W halogen illumination and movable 0.3 NA Abbe condenser for clear examination and light regulate
Oversize double-layer mechanical stage with 1.0mm stage divisions and 0.1mm vernier resolution locks slide into place and provides precise slide manipulation along the X- and Y-axes; with coaxial coarse and fine focal point

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